食管上皮癌变过程中FAS、FASL、FADD和caspase-8的表达及其意义

Objective To investigate the effects of the gene expressions of FAS、FASL、 FADD and caspase-8 in esophageal carsinogenesis. Methods Immunohistochemical method was applied to detect the expression of FAS、FASL、FADD and caspase-8 proteins in esophageal epithelium. In situ hybridization method was applied to detect the expression of FADD and caspase-8 mRNA in esophageal epithelium. Results The positive rates of FAS, FADD and caspase-8 proteins and FADD、caspase-8 mRNA were decreased from normal epithelium to dysplasia and carcinoma tissues gradually. The positive rates of FASL protein were increased from normal epithelium to dysplasia and carcinoma tissues gradually. There was very significant statistical difference between carcinoma and normal epithelium (P＜0.05). The expression of FAS、FASL and caspase-8 was correlated with the degree of tumor differentiation (P＜0.05). Conclusion The abnormal expression of FAS and FASL maybe relate with the pathogenesis of esophageal carcinoma and is also involved in the degree of differentiation and the immune escape. FADD and caspase-8 expression maybe plays an important role in esophageal carsinogenesis.