2011•Jordan Journal of Agricultural SciencesRequires access
Growth and Physiological Responses of Tomato (Lycopersicon esculentum Mill.) Callus and Cell Suspension to in Vitro Induced Salinity
Rida A. Shibli, Aktham Abu-Khadejeh, Ibrahim M. Makhadmeh, Munir J. Mohammad
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Abstract
The use of callus and cell suspension cultures to evaluate salt stress responses in two (JO112 and JO992) tomato (Lycopersicon esculentum Mill.) genotypes was determined. Callus and suspension cells were grown on media supplemented with 0, 50, 100,150 and 200 mM NaCl and CaCl2 in equal molar ratios. Callus growth, packed cell volume and cell growth (FW and DW) were significantly reduced with increasing the salt level. Na, Cl and Ca concentrations were increased in the callus with increasing salinity in both genotypes. Proline accumulation was increased with salinity increasing. Induced salinity significantly reduced cell sap osmotic potential in the callus. The results obtained in this study suggest that the higher Cl , Na and Ca accumulations found in the calli were related to the higher callus dehydration by salinity. On the other hand, K content decreased with increasing salinity.