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Sequencing the Genome

Zhanjiang Liu

Open publisher page 4 citations

Abstract

This chapter contains section titled: Strategies for Whole Genome Sequencing Whole Genome Shotgun (WGS) Hierarchical Shotgun Sequencing (the Clone-by-Clone Approach) The Hybrid Approach Selection of Minimal Tiling Path Clones Emerging Sequence Technologies and Platforms Sequencing the Genome Genome Sequence Assembly Draft Genome Sequence and Finishing Gap Filling Using Expressed Sequence Tag (EST) Resources Gap Filling by Creating Libraries with Variable Sizes Gap Filling by Primer Walking Sequencing or by Transposon Insertion into BACs Scaffolding Sequence Annotation What is Next with A, C, G, Ts? Ethical, Legal, and Social Issues (ELSI) of Genome Conclusion

About this research paper

What this paper is about

This chapter contains section titled: Strategies for Whole Genome Sequencing Whole Genome Shotgun (WGS) Hierarchical Shotgun Sequencing (the Clone-by-Clone Approach) The Hybrid Approach Selection of Minimal Tiling Path Clones Emerging Sequence Technologies and Platforms Sequencing the Genome Genome Sequence Assembly Draft Genome Sequence and Finishing Gap Filling Using Expressed Sequence Tag (EST) Resources Gap Filling by Creating Libraries with Variable Sizes Gap Filling by Primer Walking Sequencing or by Transposon Insertion into BACs Scaffolding Sequence Annotation What is Next with A, C, G, Ts? Ethical, Legal, and Social Issues (ELSI) of Genome Conclusion

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OpenAlex reports 4 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

This chapter contains section titled: Strategies for Whole Genome Sequencing Whole Genome Shotgun (WGS) Hierarchical Shotgun Sequencing (the Clone-by-Clone Approach) The Hybrid Approach Selection of Minimal Tiling Path Clones Emerging Sequence Technologies and Platforms Sequencing the Genome Genome Sequence Assembly Draft Genome Sequence and Finishing Gap Filling Using Expressed Sequence Tag (EST) Resources Gap Filling by Creating Libraries with Variable Sizes Gap Filling by Primer Walking Sequencing or by Transposon Insertion into BACs Scaffolding Sequence Annotation What is Next with A, C, G, Ts? Ethical, Legal, and Social Issues (ELSI) of Genome Conclusion

Key concepts: Shotgun sequencing, Genome, Hybrid genome assembly, Sequence assembly, Biology, Genetics, Transposable element, Whole genome sequencing

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