2009•Pesticide Research JournalRequires access

Insect growth inhibition and antifeedant activity of culture filtrate extracts of Bacillus licheniformis.

Poonam Sharma, P. Dureja, Mayurika Goel, R.S. Tanwar, J.M. Haider, Swaran Dhingra, PL Uniyal

Abstract

Cell free culture filtrate of Bacillus licheniformis isolated from rhizosphere soil of a healthy tomato plant was extracted with ethanol, methanol, ethyl acetate, dichloromethane and hexane. After removal of solvents these extracts were tested for antifeedant and insect growth activity against 3rd instars larvae of Helicoverpa armigera. Maximum antifeedant activity was exhibited by ethanol extract (Al50= 0.019%) and minimum by hexane extract (0.088%). The larval mortality ranged from 10.0–70.0% in different extracts. Two polar metabolites separated from ethanol extract showed Al50 of 0.015 and 0.053%.

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Key concepts: Helicoverpa armigera, Bacillus licheniformis, Ethyl acetate, Instar, Ethanol, Dichloromethane, Biology, Hexane