PCR 2 : a practical approach
Michael J. McPherson, Philip Quirke, G R Taylor
Abstract
Michael J. McPherson, Philip Quirke, G R Taylor
Abstract
Optimizing PCR Use of specialty phosphoramidites in PCR Chemical methods for 5' non-isotopic labelling of PCR probes and primers Solid-phase PCR Solid-phase sequencing of PCR products cDNA cloning by RT - PCR Quantification of DNA and RNA by PCR PCR MIMICS: competitive DNA fragments for use in quantitative PCR In vitro expression of proteins from PCR products PCR-based approaches to human genome mapping Fingerprinting of DNA and RNA using arbitrarily primed PCR Mutational analysis Mutational analysis: new mutations Linear amplification for the in vitro study of ligand/DNA interactions Ligand-mediated PCR.
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Optimizing PCR Use of specialty phosphoramidites in PCR Chemical methods for 5' non-isotopic labelling of PCR probes and primers Solid-phase PCR Solid-phase sequencing of PCR products cDNA cloning by RT - PCR Quantification of DNA and RNA by PCR PCR MIMICS: competitive DNA fragments for use in quantitative PCR In vitro expression of proteins from PCR products PCR-based approaches to human genome mapping Fingerprinting of DNA and RNA using arbitrarily primed PCR Mutational analysis Mutational analysis: new mutations Linear amplification for the in vitro study of ligand/DNA interactions Ligand-mediated PCR.
Key concepts: Primer dimer, Molecular biology, DNA, Biology, In silico PCR, Complementary DNA, Multiple displacement amplification, RNA