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PCR 2 : a practical approach

Michael J. McPherson, Philip Quirke, G R Taylor

Open publisher page 342 citations

Abstract

Optimizing PCR Use of specialty phosphoramidites in PCR Chemical methods for 5' non-isotopic labelling of PCR probes and primers Solid-phase PCR Solid-phase sequencing of PCR products cDNA cloning by RT - PCR Quantification of DNA and RNA by PCR PCR MIMICS: competitive DNA fragments for use in quantitative PCR In vitro expression of proteins from PCR products PCR-based approaches to human genome mapping Fingerprinting of DNA and RNA using arbitrarily primed PCR Mutational analysis Mutational analysis: new mutations Linear amplification for the in vitro study of ligand/DNA interactions Ligand-mediated PCR.

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Optimizing PCR Use of specialty phosphoramidites in PCR Chemical methods for 5' non-isotopic labelling of PCR probes and primers Solid-phase PCR Solid-phase sequencing of PCR products cDNA cloning by RT - PCR Quantification of DNA and RNA by PCR PCR MIMICS: competitive DNA fragments for use in quantitative PCR In vitro expression of proteins from PCR products PCR-based approaches to human genome mapping Fingerprinting of DNA and RNA using arbitrarily primed PCR Mutational analysis Mutational analysis: new mutations Linear amplification for the in vitro study of ligand/DNA interactions Ligand-mediated PCR.

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OpenAlex reports 342 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Optimizing PCR Use of specialty phosphoramidites in PCR Chemical methods for 5' non-isotopic labelling of PCR probes and primers Solid-phase PCR Solid-phase sequencing of PCR products cDNA cloning by RT - PCR Quantification of DNA and RNA by PCR PCR MIMICS: competitive DNA fragments for use in quantitative PCR In vitro expression of proteins from PCR products PCR-based approaches to human genome mapping Fingerprinting of DNA and RNA using arbitrarily primed PCR Mutational analysis Mutational analysis: new mutations Linear amplification for the in vitro study of ligand/DNA interactions Ligand-mediated PCR.

Key concepts: Primer dimer, Molecular biology, DNA, Biology, In silico PCR, Complementary DNA, Multiple displacement amplification, RNA

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