Effects of HMW and LMW glutenin subunits from synthetic hexaploid wheat on gluten quality parameters of derivative line

In order to provide reference for improvement of gluten quality in Chinese spring wheat breeding programs,offsprings of 167 CIMMYT synthetic wheat crossing with common wheats were employed to determine the composition of HMW-GS and LMW-GS,protein content,flour wet gluten content,Zeleny sendimentation volume,and parameters of Mixograph in this study.The results showed that HMW-GS of 2*,1,7+9,5+10,and LMW-GS of Glu-A3d,Glu-B3j and Glu-B3b were the dominant glutenin subunits,and their frequencies were 49.1%,39.5%,58.7%,73.7%,39.5%,44.3% and 32.9%,respectively.Less effect of glutenin subunits and their interaction on wet gluten content was observed.According to the contribution of glutenin subunits to Zeleny sedimentation value,midline peak time,midline peak value,midline peak width and midline = 8 min value,different glutenin subunits loci could be ranked as: Glu-B3 Glu-D1 Glu-B1 Glu-A3 Glu-A1.At Glu-A1,1 2* Null;at Glu-B1,7+8=17+187+9;at Glu-D1,5+10 1.5+10 2+12;atGlu-A3,Glu-A3b Glu-A3d Glu-A3a Glu-A3e Glu-A3c;at Glu-B3,Glu-B3d Glu-B3b Glu-B3j.Lines carrying Glu-B3j,which generally associated with poor dough strength and mixing tolerance,should be discarded in wheat breeding.