2004•Unpublished venueOpen access
Exploring the Capabilities of Nucleic Acid Polymerases by Use of Directed Evolution
Susanne Brakmann, Marina Schlicke, Petra Tafelmeyer, Kai Johnsson
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Abstract
This chapter contains sections titled: Introduction Directed Evolution of Nucleic Acid Polymerases Practical Approaches to the Directed Evolution of Polymerase Function: Selection or Screening? Selection of Polymerases with Altered Activity and Fidelity Screening Polymerase Libraries for Altered Activity Genetic Selection of an Error-prone Variant of Bacteriophage T7 RNA Polymerase Screening for Polymerases with Altered Substrate Tolerance Alternative Scenarios for Assaying Polymerase Activity Concluding Remarks References Directed Molecular Evolution of Proteins