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Quantitative Measurement of Cell Adhesion Using Centrifugal Force

David R. McClay, Philip L. Hertzler

Abstract

In order to study the biophysical forces involved in cell-substrate (or cell-cell) adhesion, it is necessary to measure the strength of adhesion. Two questions can be addressed using the centrifugal cell adhesion assay provided in this unit: what is the ligand-receptor affinity for cells adhering at 4 degrees C and what is the strength of the ATP-dependent processes that strengthen adhesion at 37 degrees C. In both cases, the strength of adhesion is measured as the resistance to disruption in the presence of a measured centrifugal force.

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Key concepts: Adhesion, Cell adhesion, Centrifugal force, Biophysics, Substrate (aquarium), Cell, Chemistry, Materials science