1973•Journal of Biological ChemistryOpen access

Cytochalasin B-sensitive 2-Deoxy-d-glucose Transport in Adipose Cell Ghosts

Michael Czech, David G. Lynn, William S. Lynn

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Abstract

Glucose oxidation of isolated white fat cells in the presence or absence of insulin was abolished by as little as 3 pM cytochalasin B in medium containing 0.2 mM glucose.This agent inhibited fat cell D-Il-Wlglucose conversion to COa half-maximally at 0.2 pM, which was about 700 and 30 times as effective as phlorizin and phloretin, respectively.

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Glucose oxidation of isolated white fat cells in the presence or absence of insulin was abolished by as little as 3 pM cytochalasin B in medium containing 0.2 mM glucose.This agent inhibited fat cell D-Il-Wlglucose conversion to COa half-maximally at 0.2 pM, which was about 700 and 30 times as effective as phlorizin and phloretin, respectively.

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Available abstract

Glucose oxidation of isolated white fat cells in the presence or absence of insulin was abolished by as little as 3 pM cytochalasin B in medium containing 0.2 mM glucose.This agent inhibited fat cell D-Il-Wlglucose conversion to COa half-maximally at 0.2 pM, which was about 700 and 30 times as effective as phlorizin and phloretin, respectively.

Key concepts: Phloretin, Phlorizin, Cytochalasin B, Adipose tissue, Glucose transporter, Chemistry, White adipose tissue, Cell

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