TaqMan® Technology and Real‐Time Polymerase Chain Reaction
John O’Leary, Orla Sheils, Cara Martin, Aoife Crowley
Abstract
John O’Leary, Orla Sheils, Cara Martin, Aoife Crowley
Abstract
This chapter contains sections titled: Introduction Probe Technologies TaqMan® Probe and Chemistry (First Generation) Second Generation TaqMan® Probes Hybridisation TaqMan® PCR Conditions Standards for Quantitative PCR Interpretation of Results End-point Detection Real-time Detection Relative Quantitation Reference Genes Specific TaqMan® PCR Applications References
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This chapter contains sections titled: Introduction Probe Technologies TaqMan® Probe and Chemistry (First Generation) Second Generation TaqMan® Probes Hybridisation TaqMan® PCR Conditions Standards for Quantitative PCR Interpretation of Results End-point Detection Real-time Detection Relative Quantitation Reference Genes Specific TaqMan® PCR Applications References
Key concepts: TaqMan, Real-time polymerase chain reaction, Polymerase chain reaction, Computational biology, Molecular biology, Biology, Gene, Chemistry