1981•Journal of BacteriologyOpen access

Transformation of Bacillus thuringiensis subsp. galleria protoplasts by plasmid pBC16

S. I. Alikhanian, N. F. Ryabchenko, Nickolai O. Bukanov, Vehary A. Sakanyan

Abstract

Protoplasts of the entomopathogenic bacterium Bacillus thuringiensis subsp. galleria were transformed by plasmid pBC16. The frequency of transformation was much lower than that of Bacillus subtilis. All isolated B. thuringiensis transformants were characterized by increased sensitivity to lysozyme as compared with the original strain.

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Key concepts: Bacillus thuringiensis, Biology, Protoplast, Bacillus subtilis, Transformation (genetics), Plasmid, Microbiology, Bacillaceae