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2009•Asian Journal of Research in ChemistryRequires access

RP-HPLC Determination of Atenolol in Its Pharmaceutical Dosage Forms

Amrish Sharma, Mukul Tailang, Sonali Khare, Tripti Shukla

Open publisher page 1 citations

Abstract

A new and simple reverse phase HPLC method was developed for the estimation of atenolol in its pharmaceutical dosage forms. The mobile phase used acetonitrile, methanol, KH2PO4 is in the ratio of 250:250:500. Buffer solution was prepared by dissolving 0.05M KH2PO4 + 0.1% H3PO4 adjunct with triethylamine. The separation was achieved on hypersil C-18 column, phenomenex Gemini(250× 4.6mm) and 5μ particle size with rheodyne injector. The flow rate was 1ml/min and uv detection at 238nm. The retention time for atenolol was 4min respectively. The linearity coefficient of atenolol was found to be 0.99% and the percentage recoveries for atenolol is 99.89%. The proposed method was accurate, precise and linear within the desired range. This method can successfully employed for the quantitative analysis of atenolol.

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What this paper is about

A new and simple reverse phase HPLC method was developed for the estimation of atenolol in its pharmaceutical dosage forms. The mobile phase used acetonitrile, methanol, KH2PO4 is in the ratio of 250:250:500. Buffer solution was prepared by dissolving 0.05M KH2PO4 + 0.1% H3PO4 adjunct with triethylamine. The separation was achieved on hypersil C-18 column, phenomenex Gemini(250× 4.6mm) and 5μ particle size with rheodyne injector. The flow rate was 1ml/min and uv detection at 238nm. The retention time for atenolol was 4min respectively. The linearity coefficient of atenolol was found to be 0.99% and the percentage recoveries for atenolol is 99.89%. The proposed method was accurate, precise and linear within the desired range. This method can successfully employed for the quantitative analysis of atenolol.

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Available abstract

A new and simple reverse phase HPLC method was developed for the estimation of atenolol in its pharmaceutical dosage forms. The mobile phase used acetonitrile, methanol, KH2PO4 is in the ratio of 250:250:500. Buffer solution was prepared by dissolving 0.05M KH2PO4 + 0.1% H3PO4 adjunct with triethylamine. The separation was achieved on hypersil C-18 column, phenomenex Gemini(250× 4.6mm) and 5μ particle size with rheodyne injector. The flow rate was 1ml/min and uv detection at 238nm. The retention time for atenolol was 4min respectively. The linearity coefficient of atenolol was found to be 0.99% and the percentage recoveries for atenolol is 99.89%. The proposed method was accurate, precise and linear within the desired range. This method can successfully employed for the quantitative analysis of atenolol.

Key concepts: Atenolol, Chromatography, Dosage form, Triethylamine, Chemistry, Dissolution, High-performance liquid chromatography, Retention time

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