Effectiveness of a desensitizing dentifrice in obliterating dentinal tubules: an in vitro study

"The most widely used method for treatment of dentin hypersensitivity is daily brushing with desensitizing dentifrices, particularly those containing active ingredients capable of obliterating the dentinal tubules, such as arginine. The present study sought to assess the action of a desensitizing dentifrice on dentin in vitro. Twenty dentin samples were obtained from 10 sound, freshly extracted third molars. The dentin specimens were distributed across four groups: group 1, one application of the dentifrice only, using a finger and rubbing for 1 minute; group 2, two daily applications of the dentifrice, by brushing, for 2 days; group 3, two daily applications of the dentifrice, by brushing, for 3 days; and group 4, no treatment. Samples were analyzed by scanning electron microscopy. There was no obliteration of dentinal tubules in the specimens from group 1. In group 2, partial or complete obliteration of dentinal tubules was observed in all specimens. In group 3, complete obliteration of tubules was observed in all specimens. We conclude that twice-daily application of the tested dentifrice by brushing, for 2 days or longer, led to obliteration of dentinal tubules. This effect may contribute to a reduction in dentin hypersensitivity."