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RESEARCH CONCERNING THE INFLUENCE OF CAPACITATION CONDITIONS ON THE QUALITY OF SPERMATOZOA USED FOR IN VITRO FERTILIZATION

Marius Zăhan, V. MICLEA, I. ROMAN, Ileana Miclea, Andrea Hettig

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Abstract

The optimization of capacitation technique is based on the study of the influence factors of this process. Studying the influence of capacitation conditions on boar sperm quality we concentrated our research upon capacitation technique and method. We used a dilution and washing method (centrifugation), a migration method (swim-up) and a selective washing method (Percoll). The capacitation medium acts and interacts with the capacitation technique and in order to study these factors we used: Tyrode`s, TALP-HEPES and TCM 199 media. The results showed that the capacitation technique influences the parameters of the sperm used for embryo in vitro production and the technique depends on the quality of the raw semen.

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What this paper is about

The optimization of capacitation technique is based on the study of the influence factors of this process. Studying the influence of capacitation conditions on boar sperm quality we concentrated our research upon capacitation technique and method. We used a dilution and washing method (centrifugation), a migration method (swim-up) and a selective washing method (Percoll). The capacitation medium acts and interacts with the capacitation technique and in order to study these factors we used: Tyrode`s, TALP-HEPES and TCM 199 media. The results showed that the capacitation technique influences the parameters of the sperm used for embryo in vitro production and the technique depends on the quality of the raw semen.

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Available abstract

The optimization of capacitation technique is based on the study of the influence factors of this process. Studying the influence of capacitation conditions on boar sperm quality we concentrated our research upon capacitation technique and method. We used a dilution and washing method (centrifugation), a migration method (swim-up) and a selective washing method (Percoll). The capacitation medium acts and interacts with the capacitation technique and in order to study these factors we used: Tyrode`s, TALP-HEPES and TCM 199 media. The results showed that the capacitation technique influences the parameters of the sperm used for embryo in vitro production and the technique depends on the quality of the raw semen.

Key concepts: Capacitation, Human fertilization, In vitro fertilisation, Andrology, Quality (philosophy), In vitro, Biology, Cell biology

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RESEARCH CONCERNING THE INFLUENCE OF CAPACITATION CONDITIONS ON THE QUALITY OF SPERMATOZOA USED FOR IN VITRO FERTILIZATION — Research Paper | ScholarLens