2002•华中科技大学学报：医学英德文版Requires access
Apoptosis of Leukemia Cells Induced by CD34^＋ Cells Transferred Exogenous Fas Ligand
肖娟, 邹萍, 刘忠文, 胡中波, 刘凌波
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Abstract
Summary: To assess the value of CD34+ cells transferred exogenous Fas ligand (FasL) in inducing apoptosis of human leukemic cells, the CD34+ cells transfected with FasL or without, pretreated with mitomycin C, was mixed with leukemic cell line U937 cells in presence or absence of daunorubicin (DNR) or cytosine arabinoside (Ara-C). After 18 h, apoptosis of cells was detected by FCM and TUNEL. Induced for 18 h by CD34+ cells transfected with FasL or without, the ratio of apoptosis of U937 cells was (5. 0±1.3) %, (10. 8±0. 6) % (P＜ 0. 01), respectively. Induced by FasL+CD34++DNR, FasL+CD34++Ara-C, the ratio was (13.4±1.0) % (P＜ 0. 05), (17.9±1.3)% (P＜0. 01), respectively. The result demonstrated that CD34+ cells transfected with exogenous FasL could induce apoptosis of human leukemic cells and showed a cytotoxic synergistic effect when used in combination with chemotherapeutic drugs, suggesting that it was possible to develop a new method in treatment of leukemia.