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1970•Journal of Biological ChemistryOpen access

Enzymatic Conversion of Linoleic Acid to 10d-Hydroxy-Δ12-cis-octadecenoic Acid

George J. Schroepfer, Walter G. Niehaus, James A. McCloskey

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Abstract

Abstract A soluble enzyme preparation from a pseudomonad was shown to catalyze the conversion of linoleic acid to 10d-hydroxy-cis-Δ12-octadecenoic acid. The assignment of structure was based upon chromatographic studies, infrared spectroscopy, optical rotation studies, and, to a major extent, mass spectrometry, including combined gas-liquid chromatography-mass spectrometry, high resolution mass spectrometry, and the preparation of deuterium-labeled derivatives prior to mass spectrometry.

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What this paper is about

Abstract A soluble enzyme preparation from a pseudomonad was shown to catalyze the conversion of linoleic acid to 10d-hydroxy-cis-Δ12-octadecenoic acid. The assignment of structure was based upon chromatographic studies, infrared spectroscopy, optical rotation studies, and, to a major extent, mass spectrometry, including combined gas-liquid chromatography-mass spectrometry, high resolution mass spectrometry, and the preparation of deuterium-labeled derivatives prior to mass spectrometry.

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Available abstract

Abstract A soluble enzyme preparation from a pseudomonad was shown to catalyze the conversion of linoleic acid to 10d-hydroxy-cis-Δ12-octadecenoic acid. The assignment of structure was based upon chromatographic studies, infrared spectroscopy, optical rotation studies, and, to a major extent, mass spectrometry, including combined gas-liquid chromatography-mass spectrometry, high resolution mass spectrometry, and the preparation of deuterium-labeled derivatives prior to mass spectrometry.

Key concepts: Linoleic acid, Enzyme, Chemistry, Biochemistry, Fatty acid

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