Ploidy analysis of Carassius auratus auratus, allogynogenetic silver crucian carp, and multiple tetraploid allogynogenetic crucian carp.

The karyotype,morphology differences of erythrocyte and nuclei were compared in Carassius auratus auratus(2n),allogynogenetic silver crucian carp Carassius auratus gibelio(3n),and multiple tetraploid allogynogenetic crucian carp.The results showed:(1) Carassius auratus auratus has 2n = 100 diploid chromosomes and the karyotype of 22 metacentric,34 submetacentric,22 subtelocentric and 22 telocentric,NF = 156.The allogynogenetic silver crucian carp has 156 chromosomes in its karyotype,with 33 metacentric,51 submetacentric,33 subtelocentric,33 telocentric,and 6 supernumerary microchromosomes.The karyotype of the multiple tetraploid allogynogenetic crucian carp contains 206 chromosomes,with 44 metacentric,68 submetacentric,44 subtelocentric,44 telocentric,and 6 supernumerary microchromosomes.(2) The long diameter,the short diameter,and the volume of erythrocyte nuclei increase significantly(P0.05) according to the ploidy of chromosome sets in 3 crucian carps.Among them,the erythrocyte nuclear volume in the multiple tetraploid allogynogenetic crucian carp and allogynogenetic silver crucian carp 3n increases extremely,which is 1.97 and 1.65 times as that of diploid crucian carp.By using Coulter counter particle analysis system,3 different ploidy crucian carps can be examined rapidly.(3) Some abnormal erythrocyte nuclei(about 10%) are observed in the multiple tetraploid allogynogenetic crucian carp.