Purification, cultivation characteristic and degrading-pathway on nitrobenzene degradation bacterium

Microbial removal is the advanced method to deal with contamination caused by nitrobenzene. This study purified nitrobenzene degradation bacterium and to get its optimum conditions of growth and degrading-pathway. Samples were collected from sediment of the outfall of a chemical factory. Bio-reduction experiments were conducted in different concentrations of nitrobenzene, temperature and pH. The reaction mixtures were analyzed by Nuclear Magnetic Resonance. Four pairs of prior strains which can directly reduce nitrobenzene were isolated. They are able to utilized nitrobenzene as the sole carbon source, which was identified as: Planococcus Migula and Pseudomonas Migula. The results show that the optimum growth conditions of nitrobenzene degradation bacterium: 25°C∼35°C, pH 7∼8 and nitrobenzene concentration at 400mg/L. With analyzed by NMR, it was found that the reaction mixtures was degraded by nitrobenzene-degrading strains over 5 days and new substance were produced.