Mammalian Artificial Chromosomes: Methods And Protocols

From Natural to Artificial Chromosomes: An Overview Vittorio Sgaramella and Sandro Eridani Methodologies Applied to Domestic Animal Chromosomes Leopoldo Iannuzzi Mapping of the Hotspots of Recombination in Human DNA Cloned as Yeast Artificial Chromosomes Grzegorz Ira, Jacek Krol, and Jan Filipski Genomic Imprinting and Its Effects on Genes and Chromosomes in Mammals Yuji Goto and Robert Feil Centromeres and Neocentromeres Gerard Roizes, Christoph Grunau, Jerome Buard, Albertina De Sario, and Jacques Puechberty Identification of Start Sites of Bi-Directional DNA Synthesis at Eukaryotic DNA Replication Origins Alessandro Vindigni, Gulnara Abdurashidova, and Arturo Falaschi Telomere Length Analysis and In Vitro Telomerase Assay Fiorentina Ascenzioni, Pier Assunta Fradiani, and Pierluigi Donini Telomerization of Mammalian Cells and Transplantation of Telomerized Cells in Immunodeficient Mice Peter J. Hornsby Chromosome-Based Vectors for Mammalian Cells: An Overview Han N. Lim and Christine J. Farr Mammalian Artificial Chromosome Formation in Human Cells After Lipofection of a PAC Precursor Jose I. de las Heras, Leonardo D'Aiuto, and Howard Cooke Use of Natural and Artificial Chromosome Vectors for Animal Transgenesis Yoshimi Kuroiwa, Kazuma Tomizuka, and Isao Ishida Pronuclear Microinjection of Purified Artificial Chromosomes for Generation of Transgenic Mice: Pick-and-Inject Technique Diane P. Monteith, Josephine D. Leung, Anita H. Borowski, Deborah O. Co, Tunde Praznovszky, Frank R. Jirik, Gyula Hadlaczky, and Carl F. Perez Modification of Human Bacterial Artificial Chromosome Clones for Functional Studies and Therapeutic Applications Michael R. Orford, Duangporn Jamsai, Samuel McLenachan, and Panayiotis A. Ioannou Index