1982•Applied and Environmental MicrobiologyOpen access

Simple method for quantitation of viable mycoplasmas

A C Albers, R. D. Fletcher

Abstract

A rapid, simple, and inexpensive method for quantitation of viable mycoplasmas is described. Serial dilutions were made in sterile microtiter plates with standard microtiter equipment. The results were multiplied by a factor of 2.38 to obtain colony-forming units comparable to those obtained with the laborious glass pipette-tube dilution method.

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Available abstract

Key concepts: Pipette, Serial dilution, Microtiter plate, Chromatography, Simple (philosophy), Dilution, Chemistry, Biology