1986•Journal of BacteriologyOpen access

Stage-specific DNA methylation in a fungal plant pathogen

Eldon R. Jupe, Jane M. Magill, Clint Magill

Abstract

Significantly more 5-methylcytosine residues were found in the DNA from the dormant sclerotia of Phymatotrichum omnivorum than in the DNA from the metabolically active mycelia of the fungus, as shown by high-pressure liquid chromatography of acid-hydrolyzed DNA digests and by restriction of the DNA with the isoschizomers MspI and HpaII. N6-Methyladenine was not detected in GATC sequences in the DNA isolated from either stage.

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Key concepts: Isoschizomer, Biology, HpaII, DNA, 5-Methylcytosine, DNA methylation, Biochemistry, Methylation