Cross-talk between NF-kB and Neuronal Nuclei (NeuN) in HT 22 mouse hippocampus neuron: a mechanism for neuronal survival versus damage reaction (54.19)

Abstract The Neuronal Nuclei or NeuN is well known as a marker for post mitotic neuron cell body in central nervous system of fetal as well as adult brain. However, functional role of NeuN has not been well elucidated yet. The present study demonstrated a co-association between NF-kB and NeuN in HT22 mouse hippocampus neuron cell. We found that, intracellular TLR7/8 ligand imidazoquinoline compound induced NF-kB activation in HT22 cells. The nuclear extracts of HT22 cells were found to demonstrate NeuN as well as NF-kB. Further, co-immunoprecipitation of NeuN from nuclear extracts of imidazoquinoline treated HT 22 hippocampus cells demonstrated an association with NF-kB. The observations suggested an interaction between NeuN and NF-kB in HT22 hippocampus neuron. A study has been initiated to determine a regulatory role of NeuN in management of neuronal dysfunction during dementia and epileptic seizures. Acknowledgement. The HT22 cells were provided by Dr Mark S Kindy (MUSC). We are thankful to Eileen McFadden for her support.