IN-VITRO MICROPROPAGATION AND REGENERATION OF TURMERIC

Micropropagation and Regeneration of BY Hajare Sunil Tulshiram ABSTRACT A study on In-vitro regeneration and micropropagation of turmericp with two cultivars viz, Rajapuri and Shialam was conducted in the Department of Biotechnology, College of Agriculture, Raipur (C.G.), with the following major objectives. 1. development of efficient in-vitro regeneration system in turmeric, 2. study the effect of genotypes on in-vitro regeneration and 3. to study different combinations and concentrations of growth hormones for efficient tissue culture. For regeneration of plant rhizome buds were used as explants. Best shoot initiation was obtained from rhizome bud explants of turmeric on MS medium supplemented with 2.0 mg/1 BAP. The combination of BAP + kinetin didnmproduce any additional positive effect for shoot initiation. The initiated shoots increased two to three folds upon subculture on the medium containing 1.0 mg/1 B/vP + 3.0 mg/1 kinetin and 2.0 mg/1 BAP-r 2.0 ing/1 kinetin. Among these two concentrations, 2.0 mg/1 BAP + 2.0 mg/1 kinetin was proved better for shoot multiplication. The in-vitro response was better in Shialam. Rooting percentage and number of roots/ shoot was found best on MS medium containing 1.0 mg/1 IB A + 5.0 mg/1 activated charcoal. After rooting, the plants were hardened and acclimatized in sand. The trays were covered with polythene sheets. Later, the in-vitro regenerated plants were transferred to kj-- the pot containing mixture of soil + FYM + sand and finally to ^filoph-JThe sprvival rate of the in-vitro regenerated plantlets was 82.66%. (Chairman of advisory committee)