2003•Current Protocols in Cell BiologyRequires access
Preparation of Human Epidermal Keratinocyte Cultures
Susan S. Yamada
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Abstract
Cultured keratinocytes have been used by a number of investigators in studies investigating wound repair and carcinogenesis, and they have also proven useful as a model for differentiation. This unit describes a protocol for establishing human keratinocytes in tissue culture. Human newborn foreskins are proteolytically digested to separate the epidermis and the dermis, and keratinocytes are obtained from the epidermis.