ISOLATION AND PURIFICATION OF ACTIVE COMPONENTS FROM THE CHINESE MEDICINAL HERB POLYGONUM CUSPIDATUM SIEB.ET ZUCC BY ADSORPTION CHROMATOGRAPHY ON 12% CROSS-LINKED AGAROSE GEL MEDIA

A chromatographic method for isolation and purification of active components from Polygonum cuspidatum Sieb. et Zucc was established by using Superose 12 as the separation media. Methanol (65%) was used as the mobile phase for separation. Four components including piceid, resveratrol, anthraglycoside B, and emodin were obtained in one-step separation. The purity of these compounds was 98.3%, 99.3%, 98.7%, and 99.1%, respectively, as determined by HPLC area normalization method. The chemical structures of these compounds were identified according to their 1H NMR and 13C NMR data.