LD Typing for Bone Marrow Transplantation.

The primary goal of this contract was to develop a support facility for HLA-D typing. The major accomplishments were: (1) Establishment of a facility for the performance of large scale sterile tissue culture; (2) Development of new methods of collecting and cryopreserving large amounts of lymphoid cells to use as reagents for HLA-D typing; (3) Development of techniques for irradiating cryopreserved lymphoid cells; (4) Identification of 47 new homozygous typing cells (HTC); (5) HLA-D typing of panel of 100 random individuals; (6) Participation in the Seventh International Histocompatibility Typing Workshop (Oxford, England, 1977); and (7) Organization of the First International Histocompatibility Typing Conference. Naval Medical Research Institutes, Bethesda, MD, 1977. (Author)