Distinguishing between Magnolia cultivars using AFLP fingerprinting
Anthony D. Mitchell, Roy Edwards, Chris Miles Frampton
Abstract
Anthony D. Mitchell, Roy Edwards, Chris Miles Frampton
Abstract
Amplified Fragment Length Polymorphism (AFLP) analysis was used to generate informative DNA fingerprints from Magnolia denudata, M. liliiflora, and putative cultivars of their hybrid cross, i.e., M. x soulangeana, M. x soulangeana 'Lennei', M. x soulangeana 'Rustica Rubra', M. x soulangeana 'Alba', M. x soulangeana 'Ruby', and M. x soulangeana 'San Jose'. A single specimen of unknown origin and representatives of the closely related M. campbellii and M. stellata were also included in the analysis. We show how this method may be used for distinguishing among cultivars.
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Amplified Fragment Length Polymorphism (AFLP) analysis was used to generate informative DNA fingerprints from Magnolia denudata, M. liliiflora, and putative cultivars of their hybrid cross, i.e., M. x soulangeana, M. x soulangeana 'Lennei', M. x soulangeana 'Rustica Rubra', M. x soulangeana 'Alba', M. x soulangeana 'Ruby', and M. x soulangeana 'San Jose'. A single specimen of unknown origin and representatives of the closely related M. campbellii and M. stellata were also included in the analysis. We show how this method may be used for distinguishing among cultivars.
Key concepts: Amplified fragment length polymorphism, Cultivar, DNA profiling, Biology, Botany, Genetic diversity, Genetics, Population