Inga Mahler, H. O. Halvorson
Abstract
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A hybrid molecule constructed from Escherichia coli plasmid pMB9 and a fragment of Bacillus subtilis 168 deoxyribonucleic acid functions in cells of leu-E. coli, converting them to leucine prototrophy, but fails to survive in strains of B. subtilis 168.
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A hybrid molecule constructed from Escherichia coli plasmid pMB9 and a fragment of Bacillus subtilis 168 deoxyribonucleic acid functions in cells of leu-E. coli, converting them to leucine prototrophy, but fails to survive in strains of B. subtilis 168.
Key concepts: Bacillus subtilis, Escherichia coli, Plasmid, Biology, Transformation (genetics), Auxotrophy, Hybrid plasmid, Plasmid preparation