2006•Journal of Applied Animal ResearchRequires access

Evaluation of Genetic Variability in Ponwar Cattle by Microsatellite Markers

Rekha Sharma, Alok Pandey, Yatender Singh, B. Prakash

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Abstract

Sharma, R., Pandey, A.K., Singh, Y. and Prakash, B. 2006. Evaluation of genetic variability in Ponwar cattle by microsatellite markers. J. Appl. Anim. Res., 30: 63–67. This study was conducted to determine the genetic variation of Ponwar cattle, a draft purpose breed, presently in small number. A total of 141 alleles were detected by 24 microsatellite markers. Microsatellites were highly polymorphic with mean allelic number 5.875±1.727 (ranging from 3–10 per locus). Observed heterozygosity, expected heterozygosity and observed and effective number of alleles were used to estimate the genetic variation of the breed. The observed heterozygosity in the population was 0.497±0.177, reflecting less genetic variation in this population. Population showed inbreeding (f=0.262) and heterozygote deficit. Genetic bottleneck was absent. The study revealed that breed needs immediate genetic management for the conservation and improvement.

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Sharma, R., Pandey, A.K., Singh, Y. and Prakash, B. 2006. Evaluation of genetic variability in Ponwar cattle by microsatellite markers. J. Appl. Anim. Res., 30: 63–67. This study was conducted to determine the genetic variation of Ponwar cattle, a draft purpose breed, presently in small number. A total of 141 alleles were detected by 24 microsatellite markers. Microsatellites were highly polymorphic with mean allelic number 5.875±1.727 (ranging from 3–10 per locus). Observed heterozygosity, expected heterozygosity and observed and effective number of alleles were used to estimate the genetic variation of the breed. The observed heterozygosity in the population was 0.497±0.177, reflecting less genetic variation in this population. Population showed inbreeding (f=0.262) and heterozygote deficit. Genetic bottleneck was absent. The study revealed that breed needs immediate genetic management for the conservation and improvement.

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Available abstract

Sharma, R., Pandey, A.K., Singh, Y. and Prakash, B. 2006. Evaluation of genetic variability in Ponwar cattle by microsatellite markers. J. Appl. Anim. Res., 30: 63–67. This study was conducted to determine the genetic variation of Ponwar cattle, a draft purpose breed, presently in small number. A total of 141 alleles were detected by 24 microsatellite markers. Microsatellites were highly polymorphic with mean allelic number 5.875±1.727 (ranging from 3–10 per locus). Observed heterozygosity, expected heterozygosity and observed and effective number of alleles were used to estimate the genetic variation of the breed. The observed heterozygosity in the population was 0.497±0.177, reflecting less genetic variation in this population. Population showed inbreeding (f=0.262) and heterozygote deficit. Genetic bottleneck was absent. The study revealed that breed needs immediate genetic management for the conservation and improvement.

Key concepts: Loss of heterozygosity, Microsatellite, Biology, Inbreeding, Breed, Genetic variability, Genetic variation, Genetics

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