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2009•Zhongguo yufang shouyi xuebaoRequires access

A real-time RT-PCR for detection of high pathogenic PRRSV.

Yang ZoneZhao, Mu AnXiong, Weihuan Fang

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Abstract

A real-time reverse transcription-polymerase chain reaction(real-time RT-PCR) assay based on SYBR green I was developed to rapidly detect high-pathogenic PRRSV(HP-PRRSV).The assay was based on primers derived from highly conserved regions of the Nsp2 gene of HP-PRRSV,and was optimized to obtain high specificity and sensitivity.The detection limit of the assay was 100 copies per reaction.The real-time RT-PCR assay was simple and highly reproducible,and is suitable for rapid and quantitative detection of HP-PRRSV.

About this research paper

What this paper is about

A real-time reverse transcription-polymerase chain reaction(real-time RT-PCR) assay based on SYBR green I was developed to rapidly detect high-pathogenic PRRSV(HP-PRRSV).The assay was based on primers derived from highly conserved regions of the Nsp2 gene of HP-PRRSV,and was optimized to obtain high specificity and sensitivity.The detection limit of the assay was 100 copies per reaction.The real-time RT-PCR assay was simple and highly reproducible,and is suitable for rapid and quantitative detection of HP-PRRSV.

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Available abstract

A real-time reverse transcription-polymerase chain reaction(real-time RT-PCR) assay based on SYBR green I was developed to rapidly detect high-pathogenic PRRSV(HP-PRRSV).The assay was based on primers derived from highly conserved regions of the Nsp2 gene of HP-PRRSV,and was optimized to obtain high specificity and sensitivity.The detection limit of the assay was 100 copies per reaction.The real-time RT-PCR assay was simple and highly reproducible,and is suitable for rapid and quantitative detection of HP-PRRSV.

Key concepts: Biology, Real-time polymerase chain reaction, Detection limit, Reverse transcription polymerase chain reaction, Polymerase chain reaction, Virology, Molecular biology, SYBR Green I

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