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1997•Applied and Environmental MicrobiologyOpen access

Purification and Characterization of a Proteasome from the Hyperthermophilic Archaeon Pyrococcus furiosus

Michael Bauer, Stephan Baüer, Robert M. Kelly

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Abstract

A 640-kDa proteasome consisting of (alpha) (25-kDa) and (beta) (22-kDa) subunits, and with a temperature optimum of 95(deg)C, was purified from crude cell extracts of a hyperthermophilic archaeon, Pyrococcus furiosus. Although this is the fourth member of the kingdom Euryarchaeota (and the first hyperthermophile) found to contain a proteasome, none has been identified among the members of the kingdom Crenarchaeota.

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A 640-kDa proteasome consisting of (alpha) (25-kDa) and (beta) (22-kDa) subunits, and with a temperature optimum of 95(deg)C, was purified from crude cell extracts of a hyperthermophilic archaeon, Pyrococcus furiosus. Although this is the fourth member of the kingdom Euryarchaeota (and the first hyperthermophile) found to contain a proteasome, none has been identified among the members of the kingdom Crenarchaeota.

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Available abstract

A 640-kDa proteasome consisting of (alpha) (25-kDa) and (beta) (22-kDa) subunits, and with a temperature optimum of 95(deg)C, was purified from crude cell extracts of a hyperthermophilic archaeon, Pyrococcus furiosus. Although this is the fourth member of the kingdom Euryarchaeota (and the first hyperthermophile) found to contain a proteasome, none has been identified among the members of the kingdom Crenarchaeota.

Key concepts: Pyrococcus furiosus, Hyperthermophile, Crenarchaeota, Archaea, Euryarchaeota, Biochemistry, Proteasome, Pyrococcus horikoshii

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Purification and Characterization of a Proteasome from the Hyperthermophilic Archaeon Pyrococcus furiosus — Research Paper | ScholarLens