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1967•Chemical and Pharmaceutical BulletinOpen access

The Polysaccharide from Lupinus luteus Seed. II. Puriflcation and Properties of Two Polysaccharides.

MASASHI TOMODA, Masako Kitamura

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Abstract

The polysaccharides extracted from the seed of Lupinus luteus were fractionated with the chromatography on DEAE-cellulose, and main two fractions obtained were purified by the gel filtration on Sephadex G-50 and G-100. The each of two polysaccharides so obtained was homogeneous on gel filtration and thin-layer electrophoresis. The component carbohydrates of both of them were D-galactose, L-arabinose, D-galacturonic acid, D-xylose and L-rhamnose, and the molar ratios of these sugars in the two polysaccharides were 15 : 10 : 6 : 2 : 2 in one, and 6 : 4 : 3 : 1 : 1 in the other.

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The polysaccharides extracted from the seed of Lupinus luteus were fractionated with the chromatography on DEAE-cellulose, and main two fractions obtained were purified by the gel filtration on Sephadex G-50 and G-100. The each of two polysaccharides so obtained was homogeneous on gel filtration and thin-layer electrophoresis. The component carbohydrates of both of them were D-galactose, L-arabinose, D-galacturonic acid, D-xylose and L-rhamnose, and the molar ratios of these sugars in the two polysaccharides were 15 : 10 : 6 : 2 : 2 in one, and 6 : 4 : 3 : 1 : 1 in the other.

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Available abstract

The polysaccharides extracted from the seed of Lupinus luteus were fractionated with the chromatography on DEAE-cellulose, and main two fractions obtained were purified by the gel filtration on Sephadex G-50 and G-100. The each of two polysaccharides so obtained was homogeneous on gel filtration and thin-layer electrophoresis. The component carbohydrates of both of them were D-galactose, L-arabinose, D-galacturonic acid, D-xylose and L-rhamnose, and the molar ratios of these sugars in the two polysaccharides were 15 : 10 : 6 : 2 : 2 in one, and 6 : 4 : 3 : 1 : 1 in the other.

Key concepts: Polysaccharide, Chemistry, Rhamnose, Chromatography, Sephadex, Xylose, Arabinose, Size-exclusion chromatography

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