Прямой органогенез in vitro горечавки желтой ( Gentiana lutea L. )

Adventitious shoots and roots through the direct organogenesis in vitro from the explants of stem and root origin have been generated for plants derived from four G. lutea populations. The efficiency of regeneration was found to vary with the genotype of maternal plant, kind of explants and phytohormone content and ratio in the nutrient medium. It was revealed that to generate the regenerants from the stem and root explants the most effective was combination of 5 to 10 mg/l thidiazuron with 0.01 or 1.0 mg/l 1naphtaleneacetic acid in the MurashigeSkoog nutrient medium. The leaf explants failed to produce regenerants at all. The regeneration indices for root explants exceeded those for stem ones several times.