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2011•Methods in molecular biologyRequires access

MicroRNA Expression Analysis Using the Illumina MicroRNA-Seq Platform

Shujun J. Luo

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Abstract

Direct sequencing of RNA molecules using next-generation sequencing (NGS) technology has revolutionized the analysis of transcriptome with its massively parallel throughput and low cost. Here, we describe Illumina's microRNA-Seq, a method for sequencing microRNA using the Illumina Genome Analyzer system. The sequence data generated from this method enables direct identifying and profiling of microRNAs in any given organism. It also sheds light in understanding the biogenesis and modification of microRNA.

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What this paper is about

Direct sequencing of RNA molecules using next-generation sequencing (NGS) technology has revolutionized the analysis of transcriptome with its massively parallel throughput and low cost. Here, we describe Illumina's microRNA-Seq, a method for sequencing microRNA using the Illumina Genome Analyzer system. The sequence data generated from this method enables direct identifying and profiling of microRNAs in any given organism. It also sheds light in understanding the biogenesis and modification of microRNA.

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OpenAlex reports 22 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Direct sequencing of RNA molecules using next-generation sequencing (NGS) technology has revolutionized the analysis of transcriptome with its massively parallel throughput and low cost. Here, we describe Illumina's microRNA-Seq, a method for sequencing microRNA using the Illumina Genome Analyzer system. The sequence data generated from this method enables direct identifying and profiling of microRNAs in any given organism. It also sheds light in understanding the biogenesis and modification of microRNA.

Key concepts: microRNA, Illumina dye sequencing, Computational biology, Massive parallel sequencing, Deep sequencing, DNA sequencing, Transcriptome, Biology

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