2014•PubMedRequires access
[The expression changes in microRNA-132 in the lipopolysaccharide-induced inflammation of rat alveolar macrophages].
Liu Fen, Rong Jiang, Zeng Zhenguo, Ning Zhao, Liang Xia, Cheng Nie, Qian Kejian
Open publisher page 6 citations
Abstract
OBJECTIVES: To observe the kinetic changes in microRNA-132 (miR-132) expression in rat alveolar macrophages after lipopolysaccharide (LPS)-induced inflammation, and to investigate initially on the role of miR-132 in alveolar macrophages inflammatory response. METHODS: The rat alveolar macrophages NR8383 cultured without pyrogen in vitro were divided into blank control group and LPS (1 mg/L) stimulated 3, 6, 12, 24 hours groups. Culture supernatants and cell pellets were collected at each time point respectively. Enzyme-linked immunosorbent assay (ELISA) was used to assay the production changes in tumor necrosis factor-α (TNF-α), interleukins (IL-1β and IL-6) in the supernatant. Real-time quantitative polymerase chain reaction (PCR) was used to detect the expression of miR-132 in the cells. RESULTS: After stimulating rat alveolar macrophages with LPS, the production of TNF-α (ng/L: 364.83 ± 46.29 vs. 34.07 ± 8.62, P<0.01), IL-1β (ng/L: 153.83 ± 43.67 vs. 32.33 ± 10.62, P<0.05) and IL-6 (ng/L: 183.85 ± 43.52 vs. 42.62 ± 11.21, P<0.05) were all increased significantly at 3 hours post LPS stimulation compared with blank control group, reached the peak at 12 hours (TNF-α: 605.09 ± 57.13, IL-1β: 377.09 ± 28.55, IL-6: 558.04 ± 77.45, all P<0.01), and descended at 24 hours (TNF-α: 281.95 ± 41.61, IL-1β: 263.17 ± 51.36, IL-6: 438.74 ± 79.94) but the levels remained significantly higher than blank control group (all P<0.01). The expression of miR-132 started to rise at 3 hours after LPS stimulation compared with blank control group [(1.12 ± 0.11) folds, P=0.995], and presented a gradual increasing trend at 6, 12, 24 hours [(5.98 ± 0.65), (7.64 ± 0.53), (8.92 ± 0.83) folds, all P<0.01]. CONCLUSION: The expression of miR-132 increased gradually over time after LPS-induced inflammation of rat alveolar macrophages, suggesting that miR-132 may be involved in regulation of rat alveolar macrophages inflammatory response.