2008•Xi'nan nongye xuebaoRequires access
Determination of cyromazine and melamine in feed by RP-HPLC.
Wei Rui-cheng, Zheng Qin, Mingli Chen, Shao MingCheng, Ran Wang
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Abstract
A method was developed for detecting cyromazine and melamine residues in feed by reversed-phase high performance liquid chromatography(RP-HPLC) with DAD detection at 214 nm.Cyromazine and melamine were extracted from feed with acetonitrile containing 20 % NH4OH,centrifuged,then concentrated.A NH2 column was used.The mobile phase was acetonitrile and water (97∶3,V/V),the flow rate was 1.0 mL/min.The peak area response was linear for cyromazine and melamine between 0.025-5.0 μg/mL.Recoveries of cyromazine and melamine at fortified levels of 1.0,3.0,5.0 and 8.0 mg/kg ranged from 74.04 %-86.94 %,with relative standard deviation of 0.51 %-2.21 %.The detection limit of both compounds was 0.01 μg/mL.The method was simple,rapid and suitable for determination of cyromazine and melamine residues in feed.