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A protocol for TILLING and eco-TILLING.

Bradley J. Till, Troy Zerr, Luca Comai, Steven Henikoff

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Abstract

This chapter provides a brief overview of TILLING (Targeting Induced Local Lesion in Genomes; a novel, reverse genetics approach that combines the advantages of point mutations provided by chemical mutagenesis with the advantages of PCR-based mutational screening) and Eco-TILLING [a method that uses TILLING techniques to look for natural mutations in individuals, usually for population genetics analysis] procedures, and the potential applications of both techniques in plants. Detailed descriptions of the conduct of TILLING and Eco-TILLING are provided.

About this research paper

What this paper is about

This chapter provides a brief overview of TILLING (Targeting Induced Local Lesion in Genomes; a novel, reverse genetics approach that combines the advantages of point mutations provided by chemical mutagenesis with the advantages of PCR-based mutational screening) and Eco-TILLING [a method that uses TILLING techniques to look for natural mutations in individuals, usually for population genetics analysis] procedures, and the potential applications of both techniques in plants. Detailed descriptions of the conduct of TILLING and Eco-TILLING are provided.

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OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

This chapter provides a brief overview of TILLING (Targeting Induced Local Lesion in Genomes; a novel, reverse genetics approach that combines the advantages of point mutations provided by chemical mutagenesis with the advantages of PCR-based mutational screening) and Eco-TILLING [a method that uses TILLING techniques to look for natural mutations in individuals, usually for population genetics analysis] procedures, and the potential applications of both techniques in plants. Detailed descriptions of the conduct of TILLING and Eco-TILLING are provided.

Key concepts: TILLING, Reverse genetics, Biology, Population, Mutagenesis, Genome, Genetics, Mutation

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