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2009•Acta Horticulturae SinicaRequires access

Relationship of Hemerocallis spp. wild species and cultivars by AFLP marker.

Haili Li, Dong Li

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Abstract

The genetic diversity and relationship of 35 accessions of daylilies in China was analyzed by AFLP markers.Among the 380 polymorphic bands obtained from 7 selective primer pairs,357(93.39%) was polymorphic.The average number of DNA bands per primer pair was 51 with a range of 19-85.The polymorphic rates of per primer pair were beyond 90.0%.The average genetic similarity among all 35 accessions evaluated was 0.7039 and ranged from 0.3822 to 0.9656.Based on the UPGMA dendrogram,all the materials could be divided into three types:Early-blooming type,mid-blooming type,late-blooming type.Cultivars originated from the same country could be clustered together in the AFLP dendrogram.The results showed that AFLP approach was efficient in generating more accurate information on the genetic diversity and relationship of daylily germplasms.

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What this paper is about

The genetic diversity and relationship of 35 accessions of daylilies in China was analyzed by AFLP markers.Among the 380 polymorphic bands obtained from 7 selective primer pairs,357(93.39%) was polymorphic.The average number of DNA bands per primer pair was 51 with a range of 19-85.The polymorphic rates of per primer pair were beyond 90.0%.The average genetic similarity among all 35 accessions evaluated was 0.7039 and ranged from 0.3822 to 0.9656.Based on the UPGMA dendrogram,all the materials could be divided into three types:Early-blooming type,mid-blooming type,late-blooming type.Cultivars originated from the same country could be clustered together in the AFLP dendrogram.The results showed that AFLP approach was efficient in generating more accurate information on the genetic diversity and relationship of daylily germplasms.

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Available abstract

The genetic diversity and relationship of 35 accessions of daylilies in China was analyzed by AFLP markers.Among the 380 polymorphic bands obtained from 7 selective primer pairs,357(93.39%) was polymorphic.The average number of DNA bands per primer pair was 51 with a range of 19-85.The polymorphic rates of per primer pair were beyond 90.0%.The average genetic similarity among all 35 accessions evaluated was 0.7039 and ranged from 0.3822 to 0.9656.Based on the UPGMA dendrogram,all the materials could be divided into three types:Early-blooming type,mid-blooming type,late-blooming type.Cultivars originated from the same country could be clustered together in the AFLP dendrogram.The results showed that AFLP approach was efficient in generating more accurate information on the genetic diversity and relationship of daylily germplasms.

Key concepts: UPGMA, Dendrogram, Amplified fragment length polymorphism, Genetic diversity, Biology, Cultivar, Genetic relationship, Primer (cosmetics)

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Relationship of Hemerocallis spp. wild species and cultivars by AFLP marker. — Research Paper | ScholarLens