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2005•Unpublished venueRequires access

Callus induction and organogenesis from various explants in Vigna radiata (L.) Wilczek

Srinath Rao, Prabhavati Patil, C P Kaviraj

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Abstract

Effect of different growth regulators and their concentration on callus formation and organogenesis in various explants and callus was studied in mung bean [Vigna radiata (L.) Wilczek. 2,4-D and NAA alone or in combination with Kn supported callus induction and further growth. 2,4-D proved better than NAA and addition of Kn at 4.6 (μM/L) further enhanced the growth of callus. Organogenesis was obtained from callus, shoot tip and cotyledonary node explants on BAP medium.

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What this paper is about

Effect of different growth regulators and their concentration on callus formation and organogenesis in various explants and callus was studied in mung bean [Vigna radiata (L.) Wilczek. 2,4-D and NAA alone or in combination with Kn supported callus induction and further growth. 2,4-D proved better than NAA and addition of Kn at 4.6 (μM/L) further enhanced the growth of callus. Organogenesis was obtained from callus, shoot tip and cotyledonary node explants on BAP medium.

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Available abstract

Effect of different growth regulators and their concentration on callus formation and organogenesis in various explants and callus was studied in mung bean [Vigna radiata (L.) Wilczek. 2,4-D and NAA alone or in combination with Kn supported callus induction and further growth. 2,4-D proved better than NAA and addition of Kn at 4.6 (μM/L) further enhanced the growth of callus. Organogenesis was obtained from callus, shoot tip and cotyledonary node explants on BAP medium.

Key concepts: Callus, Organogenesis, Vigna, Explant culture, Radiata, Shoot, Botany, Biology

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