2007•Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)Open access
Effect of ionizing radiation on rat peritoneal macrophages
Ana Luíza Garcia Cunha, António Clara Abreu Afonso Lourenço, João Cancela, João Castelhano, Carvalho, Sara, Saiote, Ana, Áurea A. Matias, Reis, Emília, C. W. lson Gomes. Lopes, Ana M. Santos, Botelho, Filomena
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Abstract
This study aims to evaluate the survival rate of peritoneal rat macrophages after exposure to 3 & 6 Gy ionizing radiation fields, respectively. Cells were cultured in multiwell plates (37 ºC, 5% CO2), separated in 3 groups: GI–control; GII-cells irradiated with 3 Gy; GIII–cells irradiated with 6 Gy, using a 4 MeV particle accelerator. After irradiation cells were incubated for 2h30 = T0, 13 h = T1, 20 h = T2, 36 h = T3, 70 h = T4, 90 h = T5 post-irradiation. To evaluate the cell’s viability the colorimetric MTT test has been used and colour intensity was measured by microELISA. Visual control was done with a contrast phase optical microscope. Irradiation with 6 Gy is the most harmful for these cells, showing death of the majority of them. 3 Gy is also aggressive but enables a higher survival rate. Time is also an important factor to which cell death is proportional.Our results show a decrease of living cells with time and higher doses.
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