2009•Zhongguo renshougonghuanbing zazhiRequires access

Molecular identification of the Brucella strains isolated in Fujian province.

Deng Yan-qin, Jiaxiong Wang, Lin DaiHua, Liang Chen, Linglan Wang

Abstract

AMOS-PCR and MLVA were carried out to identify the Brucella strains isolated in Fujian province,which were classified as B.melitensis biovar 2 or 3 by conventional microbiological tests.All these 3 isolates were identified to be B.melitensis by AMOS-PCR.The genetic patterns obtained by MLVA were queried in the Brucella 2007 database and clustered with B.melitensis strains.It is evident that these two molecular assays may be used as the assistant tools in the identification of Brucella strains.

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Key concepts: Multiple Loci VNTR Analysis, Brucella melitensis, Brucella, Biovar, Biology, Brucellosis, Identification (biology), Brucellaceae