2003•中华创伤杂志：英文版Requires access

Effect of decoy-oligodeoxynucleotides on expression of inflammation mediators in pMΦ cells from rats

国华, 梁华平, 吕凤林, Xiang Ming Xu, 安兵, 郝天智, 王付龙, 杨文军

Abstract

Objective: To study the effect of decoy-oligodeoxynucleotides (decoy-ODNs) in dumbbell shape with the oligodeoxynucleotide sequence similar to nuclear factor kappa B (NF-κB) cis-elements on expression of inflammation mediators in pMΦ cells from rats.Methods: With carriers of cationic liposomes, decoy-ODNs were transfected into pMΦ cells of rats. Then the inhibiting effects of the decoy-ODNs on tumor necrosis factor α (TNFα), interleukin-6 (IL-6) and IL-10 were analyzed.Results: Decoy-ODNs could decrease the expression of TNFα and IL-6 in dose-dependent fashion but had weaker inhibiting effect on IL-10. Conclusions: Decoy-ODNs targeting NF-κB can decrease the expression of inflammatory mediators in pMΦ cells from rats.

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Key concepts: Decoy, Transfection, Inflammation, Cationic liposome, Tumor necrosis factor alpha, Chemistry, Liposome, Molecular biology