Analysis of colorectal cancer by two-dimensional differential gel electrophoresis.

Objective To analyze the differential protein expression between colorectal cancer (CRC)tissue and adjacent normal mucosa by two-dimensional differential gel electrophoresis(2-D DIGE)and establish the differential expression profiles of them. Methods 6 paired samples,tumor and adjacent normal tissue,were taken from fresh resections from 6 CRC patients. The samples were analyzed by 2-D DIGE, TyphoonTM multifunctional scanner and two-dimensional differential analysis software of DeCyderTM. Results There were 315 protein spotfeatures changing in abundance across the six patient samples with statistical variance of the normal:tumor spot volume ratio(P 0.05),including 138 and 103 spot-upregulated and downregulated more than 1.5-fold,respectively. Conclusion The differential expression profiles of 2-D DIGE of CRC and adjacent normal mucosa tissues were established. Upregulated and downregulated proteins,which could be confirmed statistically,were found in CRC tissue comparing with normal mucosa.