Study on factors and mechanism affecting browning of Gerbera jamesonii in tissue culture.

The leaf of flameray gerbera was cultured with five different culture media in dark and light respectively.Results showed that the worst and lightest browning were double Fe and 1/ 2MS culture medium separately.Studies on the changes of enzymic activity and phenol content concorned with browning indicated that the POD activity descended with the increasing of browning,while the PAL activity and phenol content increased with the degree of browning increasing.To prevent browning,reagents of anti-browning should be added before POD activity descends and PAL activity and phenol content increase.