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2011•Methods in molecular biologyRequires access

Strategies for Efficient Transfection of CHO-Cells with Plasmid DNA

Renate Kunert, Karola Vorauer‐Uhl

Open publisher page 7 citations

Abstract

Stable cell lines of Chinese hamster ovary (CHO) cells are the predominant source of commercial -biopharmaceutical proteins. Because making suitable CHO cell lines is time-consuming and costly, -preliminary experiments with transient expression are usually performed to optimize as many protein -production parameters as possible. Here, we describe protocols for optimizing expression in transient expression experiments and isolating stable CHO cell lines using two types of self-made reagents, namely, lipoplexes and polyplexes.

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What this paper is about

Stable cell lines of Chinese hamster ovary (CHO) cells are the predominant source of commercial -biopharmaceutical proteins. Because making suitable CHO cell lines is time-consuming and costly, -preliminary experiments with transient expression are usually performed to optimize as many protein -production parameters as possible. Here, we describe protocols for optimizing expression in transient expression experiments and isolating stable CHO cell lines using two types of self-made reagents, namely, lipoplexes and polyplexes.

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Available abstract

Stable cell lines of Chinese hamster ovary (CHO) cells are the predominant source of commercial -biopharmaceutical proteins. Because making suitable CHO cell lines is time-consuming and costly, -preliminary experiments with transient expression are usually performed to optimize as many protein -production parameters as possible. Here, we describe protocols for optimizing expression in transient expression experiments and isolating stable CHO cell lines using two types of self-made reagents, namely, lipoplexes and polyplexes.

Key concepts: Chinese hamster ovary cell, Transfection, Plasmid, Cell culture, Cell biology, Biopharmaceutical, Transient (computer programming), Cricetulus

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