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2015•The FASEB JournalRequires access

Production of Bacterial Cellulose, Cloning and Characterization of Novel β‐glucosidases from Gluconacetobacter xylinum

Richard Auta, Joshua Loh, Iza Radecka, Paul Hooley

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Abstract

Bacterial cellulose (BC) is produced by bacteria has a unique structural and mechanical properties and is highly pure as compared to plant cellulose. In this study, BC was produced using Hestrin‐Schramm (HS) media. Two forms of cellulose were observed; a gel‐like membrane and a leathery membrane. Fourier Transform infrared spectroscopy (FTIR) investigation revealed both forms to be cellulose and indicated the presence of CH 2 and OH group at the absorption wavelength of 3300 cm ‐1 , 1650 cm ‐1 and 1025 cm ‐1 . Scanning Electron Microscopy (SEM) results showed fine morphology of the cellulose matrix and fibre; and XRD indicated the majority of the cellulose to be type‐1 cellulose (crystalline in nature). Plasmid DNA sequence analysis revealed an open reading frame of 678 bp. The significance of this work is discussed.

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What this paper is about

Bacterial cellulose (BC) is produced by bacteria has a unique structural and mechanical properties and is highly pure as compared to plant cellulose. In this study, BC was produced using Hestrin‐Schramm (HS) media. Two forms of cellulose were observed; a gel‐like membrane and a leathery membrane. Fourier Transform infrared spectroscopy (FTIR) investigation revealed both forms to be cellulose and indicated the presence of CH 2 and OH group at the absorption wavelength of 3300 cm ‐1 , 1650 cm ‐1 and 1025 cm ‐1 . Scanning Electron Microscopy (SEM) results showed fine morphology of the cellulose matrix and fibre; and XRD indicated the majority of the cellulose to be type‐1 cellulose (crystalline in nature). Plasmid DNA sequence analysis revealed an open reading frame of 678 bp. The significance of this work is discussed.

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Available abstract

Bacterial cellulose (BC) is produced by bacteria has a unique structural and mechanical properties and is highly pure as compared to plant cellulose. In this study, BC was produced using Hestrin‐Schramm (HS) media. Two forms of cellulose were observed; a gel‐like membrane and a leathery membrane. Fourier Transform infrared spectroscopy (FTIR) investigation revealed both forms to be cellulose and indicated the presence of CH 2 and OH group at the absorption wavelength of 3300 cm ‐1 , 1650 cm ‐1 and 1025 cm ‐1 . Scanning Electron Microscopy (SEM) results showed fine morphology of the cellulose matrix and fibre; and XRD indicated the majority of the cellulose to be type‐1 cellulose (crystalline in nature). Plasmid DNA sequence analysis revealed an open reading frame of 678 bp. The significance of this work is discussed.

Key concepts: Cellulose, Bacterial cellulose, Fourier transform infrared spectroscopy, Scanning electron microscope, Chemistry, Membrane, Bacteria, Nuclear chemistry

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Production of Bacterial Cellulose, Cloning and Characterization of Novel β‐glucosidases from Gluconacetobacter xylinum — Research Paper | ScholarLens