2004•Journal of Applied Biological ChemistryRequires access

PCR Detection of Listeria monocytogenes Using Specific Virulence Factor Genes

Hyun‐Joong Kim, Sang-Hoon Jung, Jonghyun Park, Geun Eog Ji, Hae‐Yeong Kim

Abstract

To investigate the novel specific genes of Listeria monocytogenes, six genes related with virulence factor of L. monocytogenes were selected from database. Primer sets were constructed from the selected six genes to evaluate the specificity of L. monocytogenes with other Listeria species. The primers were tested with 12 strains of Listeria species: L. monocytogenes (7 strains), L. welshimeri, L. innocua, L. seeligeri, L. ivanovii, and L. grayi. All primer sets showed specificity to L. monocytogenes but not with other Listeria species. These genes could be used as target genes for the detection of L. monocytogenes.

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Key concepts: Listeria monocytogenes, Virulence, Listeria, Primer (cosmetics), Microbiology, Gene, Virulence factor, Chemistry