UV Spectrophotometric Estimation of Carvedilol Hydrochloride by Second Order Derivative Methods in Bulk and Pharmaceutical Dosage Form

Simple and precise UV spectrophotometric methods by second order derivative have been developed and validated for the estimation of carvedilol hydrochloride in bulk and its tablet formulation. The standard and sample solutions of carvedilol hydrochloride were prepared in absolute alcohol. Carvedilol hydrochloride was estimated at 247.5 nm for the second order derivative UV-spectrophotometric method. Beer's law was obeyed in the concentration range of 1 to 14 μg/ml with coefficient of correlation value 0.9999 for second order derivative method. These methods were tested and validated for various parameters according to ICH guidelines. The precision expressed as relative standard deviation were of 0.03243% for the above method respectively. The proposed methods were successfully applied for the determination of carvedilol hydrochloride in pharmaceutical formulation. Results of the analysis were validated statistically and were found to be satisfactory. The proposed methods are simple, easy to apply, low-cost and require relatively inexpensive instruments.