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1985•Wood and Fiber Science (Society of Wood Science and Technology)Open access

Measuring Microfibrillar Angles Using Light Microscopy

John F. Senft, B. A. Bendtsen

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Abstract

A rapid, reliable method of enhancing microfibrillar angles for viewing in wood cell walls, a modification of I. W. Bailey's technique, is reviewed. Dried microtomed sections are treated with an iodinepotassium iodide solution and nitric acid to produce crystals in cell walls of softwood tracheids or hardwood fibers. The crystals are aligned with the predominant microfibrillar angle and may be easily viewed and measured.

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A rapid, reliable method of enhancing microfibrillar angles for viewing in wood cell walls, a modification of I. W. Bailey's technique, is reviewed. Dried microtomed sections are treated with an iodinepotassium iodide solution and nitric acid to produce crystals in cell walls of softwood tracheids or hardwood fibers. The crystals are aligned with the predominant microfibrillar angle and may be easily viewed and measured.

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Available abstract

A rapid, reliable method of enhancing microfibrillar angles for viewing in wood cell walls, a modification of I. W. Bailey's technique, is reviewed. Dried microtomed sections are treated with an iodinepotassium iodide solution and nitric acid to produce crystals in cell walls of softwood tracheids or hardwood fibers. The crystals are aligned with the predominant microfibrillar angle and may be easily viewed and measured.

Key concepts: Softwood, Hardwood, Tracheid, Materials science, Composite material, Nitric acid, Microscopy, Optics

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