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[Method of determination of proteins with Coomassie brilliant blue G 250. I. General characteristics and comparative analysis with the biuret method and Lowry's method].

S. Serra, L Morgante

Open publisher page 6 citations

Abstract

Bradford (1977) described a new sensitive easy rapid method for protein assay, depending on colour change of the stain Coomassie Brilliant Blue G 250. In this study Bradford's method was compared with the Lowry et al. 91951) and biuret methods. Coomassie method appears to be as sensitive as Lowry, as simple as biuret. Another advantage is a very short time of performing.

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What this paper is about

Bradford (1977) described a new sensitive easy rapid method for protein assay, depending on colour change of the stain Coomassie Brilliant Blue G 250. In this study Bradford's method was compared with the Lowry et al. 91951) and biuret methods. Coomassie method appears to be as sensitive as Lowry, as simple as biuret. Another advantage is a very short time of performing.

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Available abstract

Bradford (1977) described a new sensitive easy rapid method for protein assay, depending on colour change of the stain Coomassie Brilliant Blue G 250. In this study Bradford's method was compared with the Lowry et al. 91951) and biuret methods. Coomassie method appears to be as sensitive as Lowry, as simple as biuret. Another advantage is a very short time of performing.

Key concepts: Biuret test, Coomassie Brilliant Blue, Chromatography, Bradford protein assay, Lowry protein assay, Chemistry, Bicinchoninic acid assay, Staining

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[Method of determination of proteins with Coomassie brilliant blue G 250. I. General characteristics and comparative analysis with the biuret method and Lowry's method]. — Research Paper | ScholarLens